Roles of structural plasticity in chaperone HdeA activity are revealed by F-19 NMR

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Roles of structural plasticity in chaperone HdeA activity are revealed by F-19 NMR
	Zhai, Zining 1,2; Wu, Qiong 1; Zheng, Wenwen 1,2; Liu, Maili 1; Pielak, Gary J.3,4,5; Li, Conggang 1
刊名	CHEMICAL SCIENCE
	2016
卷号	7 期号:3 页码:2222-2228
英文摘要	HdeA, a minimal ATP-independent acid chaperone, is crucial for the survival of enteric pathogens as they transit the acidic (pH 1-3) environment of the stomach. Although protein disorder (unfolding) and structural plasticity have been elegantly linked to HdeA function, the details of the linkage are lacking. Here, we apply F-19 NMR to reveal the structural transition associated with activation. We find that unfolding is necessary but not sufficient for activation. Multiple conformations are present in the functional state at low pH, but the partially folded conformation is essential for HdeA chaperone activity, and HdeA's intrinsic disulfide bond is required to maintain the partially folded conformation. The results show that both disorder and order are key to function. The ability of F-19 NMR to reveal and quantify multiple conformational states makes it a powerful tool for studying other chaperones.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	ACID RESISTANCE ; ESCHERICHIA-COLI ; ENTERIC BACTERIA ; PERIPLASMIC PROTEIN ; SUBSTRATE-BINDING ; SPECTROSCOPY ; CONFORMATION ; ACTIVATION ; DOMAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000371021900075
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/9221]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ N Carolina, Dept Chem, Chapel Hill, NC USA 4.Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC USA 5.Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA
推荐引用方式 GB/T 7714	Zhai, Zining,Wu, Qiong,Zheng, Wenwen,et al. Roles of structural plasticity in chaperone HdeA activity are revealed by F-19 NMR[J]. CHEMICAL SCIENCE,2016,7(3):2222-2228.
APA	Zhai, Zining,Wu, Qiong,Zheng, Wenwen,Liu, Maili,Pielak, Gary J.,&Li, Conggang.(2016).Roles of structural plasticity in chaperone HdeA activity are revealed by F-19 NMR.CHEMICAL SCIENCE,7(3),2222-2228.
MLA	Zhai, Zining,et al."Roles of structural plasticity in chaperone HdeA activity are revealed by F-19 NMR".CHEMICAL SCIENCE 7.3(2016):2222-2228.