PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation | |
Zhijun Zhang(张智军)![]() ![]() ![]() ![]() | |
刊名 | ACS Appl. Mater. & Interfaces
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2012-11 | |
卷号 | 4期号:11页码:6317-6323 |
关键词 | graphene oxide loading capacity protein delivery cellular uptake enzymatic hydrolysis regulation of cell function |
通讯作者 | Zhijun Zhang(张智军) |
英文摘要 | Delivery of proteins into cells may alter cellular functions as various proteins are involved in cellular signaling by activating or deactivating the corresponding pathways and, therefore, can be used in cancer therapy. In this study, we have demonstrated for the first time that PEGylated graphene oxide (GO) can be exploited as a nanovector for efficient delivery of proteins into cells. In this approach, GO was functionalized with amine-terminated 6-armed polyethylene glycol (PEG) molecules, thereby providing GO with proper physiological stability and biocompatibility. Proteins were then loaded onto PEG-grafted GO (GO-PEG) with high payload via noncovalent interactions. GO-PEG could deliver proteins to cytoplasm efficiently, protecting them from enzymatic hydrolysis. The protein delivered by GO-PEG reserves its biological activity that regulates the cell fate. As a result, delivery of ribonuclease A (RNase A) led to cell death and transport of protein kinase A (PKA) induced cell growth. Taken together, this work demonstrated the feasibility of PEGlyated GO as a promising protein delivery vector with high biocompatibility, high payload capacity and, more importantly, capabilities of protecting proteins from enzymatic hydrolysis and retaining their biological functions. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000311521900080 |
公开日期 | 2013-01-22 |
内容类型 | 期刊论文 |
源URL | [http://58.210.77.100/handle/332007/1036] ![]() |
专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_张智军团队 |
通讯作者 | Zhijun Zhang(张智军); Zhijun Zhang(张智军) |
推荐引用方式 GB/T 7714 | Zhijun Zhang,Jie Huang,Jianwu Dai,et al. PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation[J]. ACS Appl. Mater. & Interfaces,2012,4(11):6317-6323. |
APA | Zhijun Zhang,Jie Huang,Jianwu Dai,&Zhijun Zhang.(2012).PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation.ACS Appl. Mater. & Interfaces,4(11),6317-6323. |
MLA | Zhijun Zhang,et al."PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation".ACS Appl. Mater. & Interfaces 4.11(2012):6317-6323. |
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