Development of CdTe/ZnSe Core/Shell Quantum Dots-based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 王强斌团队

	Development of CdTe/ZnSe Core/Shell Quantum Dots-based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol
	Hu HJ ; Fu T (付涛) ; Zhang MZ ; Hong Z ; Chen ZL ; Liu J
刊名	Chinese Journal of Analytical Chemistry
	2010-12
卷号	38 期号:12 页码:1727-1731
关键词	Cadmium telluride/zinc selenide core/shell quantum dots Clenbuterol Immunochromatography
通讯作者	Liu, J
合作状况	其它
英文摘要	CdTe/ZnSe core/shell quantum dots (QDs) were synthesized in aqueous solution by using mercaptosuccinic acid as the stabilizer, and then were coupled to the polyclonal antibody (Anti-Clenbuterol pAb, Anti-CLE pAb) with N-hydroxysuccinimide(NHS). The coupled product (CdTe/ZnSe-Anti-CLE pAb) was confirmed by agarose gel electrophoresis method, and its antibody activity was tested by protein dot-blot assay. Spectra analysis further showed that the photoluminescence (PL) intensity of the CdTe/ZnSe-Anti-CLE pAb was enhanced and the emission peak underwent a red-shift. Using the CdTe/ZnSe-Anti-CLE pAb, an immunochromatographic strip was developed for the rapid detection of clenbuterol (CLE) with a test limit of 1 mu g/L. Compared with the results of ELISA, the lateral flow strip is very useful and effective as a rapid screening method for detection of CLE residues.
收录类别	SCI
语种	英语
WOS记录号	WOS:000287782500008
公开日期	2011-03-13
内容类型	期刊论文
源URL	[http://58.210.77.100/handle/332007/359]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_王强斌团队
推荐引用方式 GB/T 7714	Hu HJ,Fu T ,Zhang MZ,et al. Development of CdTe/ZnSe Core/Shell Quantum Dots-based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol[J]. Chinese Journal of Analytical Chemistry,2010,38(12):1727-1731.
APA	Hu HJ,Fu T ,Zhang MZ,Hong Z,Chen ZL,&Liu J.(2010).Development of CdTe/ZnSe Core/Shell Quantum Dots-based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol.Chinese Journal of Analytical Chemistry,38(12),1727-1731.
MLA	Hu HJ,et al."Development of CdTe/ZnSe Core/Shell Quantum Dots-based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol".Chinese Journal of Analytical Chemistry 38.12(2010):1727-1731.