A novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene detection based on an assembly strategy of electrochemiluminescent graphene oxide with gold nanoparticles and aptamer

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 离职团队

	A novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene detection based on an assembly strategy of electrochemiluminescent graphene oxide with gold nanoparticles and aptamer
	Yu, YQ ; Cao, Q ; Zhou, M(周明) ; Cui, H*
刊名	BIOSENSORS & BIOELECTRONICS
	2013-05
卷号	43 期号:0 页码:137-142
关键词	2,4,6-trinitrotoluene Ruthenium(II) complex Graphene oxide Electrochemiluminescence Aptasensor
通讯作者	Cui, H
英文摘要	We report a novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene (TNT) detection based on an assembly strategy of electrochemiluminescent graphene oxide (GO) with gold nanoparticles (AuNPs) and aptamer. In this sensing strategy, the anti-TNT aptamer was first assembled with AuNPs to form aptamer-AuNPs. Then ruthenium(II) complex functionalized GO (denoted as Ru-GO) was assembled with aptamer-AuNPs by electrostatic interaction. AuNPs could directly quench the electrochemiluminescence (ECL) emission of ruthenium(II) complex on the surface of Ru-GO due to the energy transfer from luminophore to the AuNPs. Weak ECL signal of the assembly was obtained. In the presence of target molecule TNT, the aptamer-AuNPs would aggregate partly due to the aptamer-target interaction and reduce quenching effect, leading to ECL signal restoration and strong ECL signal was obtained. TNT in a range of 0.01-100 ng mL(-1) could be detected by use of the ECL intensity discrepancy with a low detection limit of 3.6 pg mL(-1). The aptasensor also showed high selectivity towards TNT against 2,4-dinitrotoluene, p-nitrotoluene and nitrobenzene. The present aptasensor has been successfully applied to the detection of TNT in real water samples. Compared with previous reported sensors, this homogenous aptasensor avoided complicated labeling and purification procedure and showed magnificent sensitivity and high selectivity, which made it not only convenient but also time-saving and applicable. Furthermore, this sensing strategy also provides a promising way to develop new ECL aptasensor for other analytes by virtue of other aptamers. (C) 2012 Elsevier B.V. All rights reserved.
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000316525300024
公开日期	2013-12-31
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1182]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_离职团队
推荐引用方式 GB/T 7714	Yu, YQ,Cao, Q,Zhou, M,et al. A novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene detection based on an assembly strategy of electrochemiluminescent graphene oxide with gold nanoparticles and aptamer[J]. BIOSENSORS & BIOELECTRONICS,2013,43(0):137-142.
APA	Yu, YQ,Cao, Q,Zhou, M,&Cui, H*.(2013).A novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene detection based on an assembly strategy of electrochemiluminescent graphene oxide with gold nanoparticles and aptamer.BIOSENSORS & BIOELECTRONICS,43(0),137-142.
MLA	Yu, YQ,et al."A novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene detection based on an assembly strategy of electrochemiluminescent graphene oxide with gold nanoparticles and aptamer".BIOSENSORS & BIOELECTRONICS 43.0(2013):137-142.