Assembled 3D cell niches in chitosan hydrogel network to mimic extracellular matrix

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 朱毅敏团队

	Assembled 3D cell niches in chitosan hydrogel network to mimic extracellular matrix
	Zhu, YM(朱毅敏); *Dai, JW(戴建武)**
刊名	COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
	2013-10
卷号	434 期号:78 页码:87
关键词	Extracellular matrix Layer-by-layer (LbL) molecular self-assembly Hierarchical porous structure Hydrogel scaffolds
通讯作者	Dai, JW*(戴建武)
英文摘要	Extracellular matrix (ECM) is a composite material which contains a complex mixture of fibrous proteins and heteropolysaccharides, providing an important model for designing biomaterials. To mimic ECM, we fabricated 3D cell niches in chitosan hydrogel network via the layer-by-layer (LbL) molecular self-assembly method. Compression modulus of genipin-crosslinked chitosan hydrogel could be regulated from 0.014 +/- 0.003 MPa to 0.24 +/- 0.009 MPa as chitosan concentration increased from 1 wt.% to 5 wt.%, which reached the requirements of soft tissue. The 10 mu m CaCO3 microparticles as porogens were embedded into hydrogel network to resemble in vivo 3D microstructure. Porosity could be modulated from 32.10 +/- 5.08% to 91.41 +/- 6.56% as more CaCO3 microparticles embedded. The ECM component of chondroitin sulfate (CS) was layer-by-layer assembled into hydrogel network via electrostatic interactions between negative-charged CS molecules and positive-charged chitosan molecules. Subsequently, the process of delivering fibroblast growth factor (FGF) in hydrogel network could be precisely controlled due to CS molecules' affinity interactions with FGF. In vitro 3D cell culture experiments revealed that human lung fibroblast (HLF) grew better in the scaffolds as fabricated than that in untreated chitosan hydrogel. These results suggest adaptive 3D cell niches can be fabricated through molecular self-assembly of the ECM components for engineering the aimed tissues. (C) 2013 Elsevier B.V. All rights reserved.
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000322418400011
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1198]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_朱毅敏团队苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队
通讯作者	Dai, JW*(戴建武)
推荐引用方式 GB/T 7714	Zhu, YM,Dai, JW*. Assembled 3D cell niches in chitosan hydrogel network to mimic extracellular matrix[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2013,434(78):87.
APA	Zhu, YM,&Dai, JW*.(2013).Assembled 3D cell niches in chitosan hydrogel network to mimic extracellular matrix.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,434(78),87.
MLA	Zhu, YM,et al."Assembled 3D cell niches in chitosan hydrogel network to mimic extracellular matrix".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 434.78(2013):87.