Anti-inflammatory effects of three-dimensional graphene foams cultured with microglial cells

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 程国胜团队

	Anti-inflammatory effects of three-dimensional graphene foams cultured with microglial cells
	Cheng GS(程国胜); Song Q(宋琴)
刊名	BIOMATERIALS
	2014-08
卷号	35 期号:25 页码:6930-6940
关键词	Graphene Neuroinflammation Neural stem cells Microglia
通讯作者	唐明亮
英文摘要	One of the key goals in nerve tissue engineering is to develop new materials which cause less or no neuroinflammation. Despite the rapid advances of using graphene as a neural interface material, it still remains unknown whether graphene could provoke neuroinflammation or not, and whether and how the topographical features of graphene influence the neuroinflammation induction. By immunofluorescence, Elisa technique, western blot, scanning electron microscope (SEM) methods, we investigated the pro- and/or anti-inflammatory responses of microglia in the graphene films (2D-graphene) or graphene foams (3D-graphene) culturing systems. Furthermore, the growth situations of the neural stem cells (NSCs) in the conditioned culture medium produced in the graphene substrates were evaluated. The results show that: 1) neither 2D nor 3D graphene induced distinct neuroinflammation when compared to the tissue culture polystyrene (TCPS) substrates; 2) the topographical structures of the graphene might affect the material/cell interactions, leading to disparate effects on lipopolysaccharide (LPS)-induced neuroinflammation; 3) 3D graphene exhibited a remarkable capability of rescuing LPS-induced neuroinflammation probably through the restriction of microglia morphological transformation by the unique topographical features on the surface, showing the ability of anti-inflammation against external insults, while 2D graphene failed to. These results provide insights into the diverse biological effects of the material's topographical structures and open new opportunity for the applications of graphene in neuroscience. (C) 2014 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000338386800025
公开日期	2014-12-02
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1636]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_程国胜团队
推荐引用方式 GB/T 7714	Cheng GS,Song Q. Anti-inflammatory effects of three-dimensional graphene foams cultured with microglial cells[J]. BIOMATERIALS,2014,35(25):6930-6940.
APA	Cheng GS,&Song Q.(2014).Anti-inflammatory effects of three-dimensional graphene foams cultured with microglial cells.BIOMATERIALS,35(25),6930-6940.
MLA	Cheng GS,et al."Anti-inflammatory effects of three-dimensional graphene foams cultured with microglial cells".BIOMATERIALS 35.25(2014):6930-6940.