The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin

	The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin
	Wang; Xingyong; Li YR(李玉茹); Li T(李婷); 刘蕾(多); Wu HC(吴海臣); Li; Yuru; Li; Ting
刊名	SCIENCE CHINA-CHEMISTRY
	2015
卷号	59 期号:1 页码:135-141
关键词	Biomacromolecules Current amplitude Low concentrations Secondary structures signature event Single channel recording Small organic molecules translocation
通讯作者	刘蕾（多） ; 吴海臣
英文摘要	Nanopore has been developed to be a powerful, single-molecule analytical tool for sensing ions, small organic molecules and biomacromolecules such as proteins and DNAs. Generally, the identity of the analyte can be revealed by current amplitude changes and mean dwell time of the analyte binding events. In some cases, generation of highly characteristic current events affords an alternative way of analyte determination with high confidence level. However, we found that secondary structures in DNA/RNA hybrids might severely hinder the generation of signature events during their translocation through α-hemolysin nanopore. In this report, we propose a strategy to add a certain concentration of urea in the buffer solution for single channel recordings and validate that low concentration of urea can effectively denature the secondary structures in DNA hybrids and recover the generation of signature events. This finding might be useful in other secondary structure-related nanopore sensing activities. © 2015 Science China Press and Springer-Verlag Berlin Heidelberg
类目[WOS]	461.2 Biological Materials and Tissue Engineering ; 461.9 Biology ; 761 Nanotechnology ; 804.1 Organic Compounds ; 931.3 Atomic and Molecular Physics ; 933 Solid State Physics
收录类别	EI ; CA
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/228916]
专题	高能物理研究所_多学科研究中心高能物理研究所_核技术应用研究中心
推荐引用方式 GB/T 7714	Wang,Xingyong,Li YR,et al. The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin[J]. SCIENCE CHINA-CHEMISTRY,2015,59(1):135-141.
APA	Wang.,Xingyong.,李玉茹.,李婷.,刘蕾.,...&Hai-Chen.(2015).The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin.SCIENCE CHINA-CHEMISTRY,59(1),135-141.
MLA	Wang,et al."The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin".SCIENCE CHINA-CHEMISTRY 59.1(2015):135-141.