Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans

	Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans
	Gao ZQ(高增强); Hou HF(侯海峰); Li LQ(李丽琴); Dong YH(董宇辉); Gao, ZQ; Hou, HF; Li, LQ; Rui, X; Liang, YH; Li, LF
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2007
卷号	34 期号:2 页码:#REF!
关键词	Streptococcus mutans Smu_195c crystallization X-ray diffraction
通讯作者	Dong, YH (reprint author), Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China.
英文摘要	Smu_195c is a protein with 86 amino acids in Streptococcus mutans, a primary pathogen for human dental caries. The specific function of Smu_195c is still unknown and there are no conserved domains in it. In Order to find out its function, the gene encodes Smu_195c was cloned and expressed in E. coli as N-terminally 6*His tagged recombinant protein. Two crystal forms were obtained by the hanging drop method. Form I belongs to space group P6(1)22 or P6(5)22 with the unit cell parameters a = b = 62.93 angstrom, c = 90.63 angstrom, gamma = 120 degrees and form II belongs to the space group P4(1)2(1)2 or P4(3)2(1)2 with the unit cell parameters a = b = 57.97 angstrom, c = 103.51 angstrom Crystals from the protein with His-tag belong to form I, however, crystals from the protein without His-tag belong to both.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
收录类别	SCI
WOS记录号	WOS:000244317300013
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/226916]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Gao ZQ,Hou HF,Li LQ,et al. Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34(2):#REF!.
APA	高增强.,侯海峰.,李丽琴.,董宇辉.,Gao, ZQ.,...&Dong, YH.(2007).Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,34(2),#REF!.
MLA	高增强,et al."Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 34.2(2007):#REF!.