Formation of complexes of Rubisco-Rubisco activase from La3+, Ce3+ treatment spinach

	Formation of complexes of Rubisco-Rubisco activase from La3+, Ce3+ treatment spinach
	Hong, F; Liu, C; Zheng, L; Wang, XF; Wu, K; Song, WP; Lu, SP; Tao, Y; Zhao, GW; Tao Y(陶冶)
刊名	SCIENCE IN CHINA SERIES B-CHEMISTRY
	2005
卷号	48 期号:1 页码:#REF!
关键词	La3+ Ce3+ spinach Rubisco complex of Rubisco and Rubisco acitivase spectral characteristics
通讯作者	Hong, F (reprint author), Suzhou Univ, Coll Life Sci, Suzhou 215006, Peoples R China.
英文摘要	The complex of Rubisco and Rubisco activase from LaCl3-, CeCl3- treated spinach in vivo is induced. SDS-PAGE result shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only large and small subunits (55 kD, 14.4 kD) of Rubisco, but also two large subunits of 45 kD and 41 kD near the large subunits of Rubisco. Native-PAGE shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only a band of Rubisco (560 0), but also a band of about 1100 0, about twice distant from Rubisco, which might be a complex of Rubisco and Rubisco activase. The purified enzyme activities from LaCl3-, CeCl3-treated spinach are 1.8 and 2.8 times that of the control, the intensities of absorption and fluorescence are significantly higher than that of the purified Rubisco from the control, and the total sulfhydryl groups and available sulfhydryl groups are 36-39-SH per mol enzyme, 14-25-SH per mol enzyme more than those of the purified Rubisco from the control, respectively. The CID spectra show that the secondary structure of the purified enzyme from LaCl3-, CeCl3 -treated spinach is very different from the control. The enzyme activities from LaCl3-, CeCl3-treated spinach in vivo are 1.5 and 1.9 times those of the control.
学科主题	Chemistry
类目[WOS]	Chemistry, Multidisciplinary
收录类别	SCI
WOS记录号	WOS:000228190500010
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/225897]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Hong, F,Liu, C,Zheng, L,et al. Formation of complexes of Rubisco-Rubisco activase from La3+, Ce3+ treatment spinach[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2005,48(1):#REF!.
APA	Hong, F.,Liu, C.,Zheng, L.,Wang, XF.,Wu, K.,...&陶冶.(2005).Formation of complexes of Rubisco-Rubisco activase from La3+, Ce3+ treatment spinach.SCIENCE IN CHINA SERIES B-CHEMISTRY,48(1),#REF!.
MLA	Hong, F,et al."Formation of complexes of Rubisco-Rubisco activase from La3+, Ce3+ treatment spinach".SCIENCE IN CHINA SERIES B-CHEMISTRY 48.1(2005):#REF!.