Detection of pH Change in Cytoplasm of Live Myocardial Ischemia Cells via the ssDNA-SWCNTs Nanoprobes

	Detection of pH Change in Cytoplasm of Live Myocardial Ischemia Cells via the ssDNA-SWCNTs Nanoprobes
	Liu R(刘茹); Wang YL(王亚玲); Liu, R; Liu, L; Liang, J; Wang, YL; Wei, YT; Gao, FP; Gao, L; Gao, XY
刊名	ANALYTICAL CHEMISTRY
	2014
卷号	86 期号:6 页码:3048-3052
英文摘要	Myocardial ischemia is featured by a significant increase in the cytoplasm proton concentration, and such a proton change may be applied as an index for earlier ischemic heart disease diagnostics. But such a pH change in a live heart cell is difficult to monitor as a normal fluorescent probe cannot specifically transport into the cytoplasm of an ischemic cell. This is because the heart cell contains condensed myofibrils which are tight barriers for a normal probe to penetrate. We design fluorescent probes, single-strand DNA wrapped single-wall carbon nanotubes (ssDNA-SWCNTs), where the ssDNA is labeled by the dye molecule hexachloro-6-carboxyfluorescein (HEX). This nanoprobe could transport well into a live heart cell and locate in the cytoplasm to sensitively detect the intracellular pH change of myocardial ischemia. Briefly, protons neutralize the negative charges of nanoprobes in the cytoplasm. This will weaken the stability of nanoprobes and further tune their aggregation. Such aggregations induce the HEX of some nanoprobes condensed together and further result in their fluorescence quenching. The nanoprobes are advantaged in penetrating condensed myofibrils of the heart cell, and their fluorescence intensity is sensitive to the proton concentration change in the live cell cytoplasm. This new method may provide great assistance in earlier cardiopathy diagnosis in the future.
学科主题	Chemistry
收录类别	SCI
WOS记录号	WOS:000333235700032
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/224809]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Liu R,Wang YL,Liu, R,et al. Detection of pH Change in Cytoplasm of Live Myocardial Ischemia Cells via the ssDNA-SWCNTs Nanoprobes[J]. ANALYTICAL CHEMISTRY,2014,86(6):3048-3052.
APA	刘茹.,王亚玲.,Liu, R.,Liu, L.,Liang, J.,...&高学云.(2014).Detection of pH Change in Cytoplasm of Live Myocardial Ischemia Cells via the ssDNA-SWCNTs Nanoprobes.ANALYTICAL CHEMISTRY,86(6),3048-3052.
MLA	刘茹,et al."Detection of pH Change in Cytoplasm of Live Myocardial Ischemia Cells via the ssDNA-SWCNTs Nanoprobes".ANALYTICAL CHEMISTRY 86.6(2014):3048-3052.