Anionic Lipid, pH-Sensitive Liposome-Gold Nanoparticle Hybrids for Gene Delivery - Quantitative Research of the Mechanism

	Anionic Lipid, pH-Sensitive Liposome-Gold Nanoparticle Hybrids for Gene Delivery - Quantitative Research of the Mechanism
	Du,Baoji; Tian,Li; Gu,Xiaoxiao; Li,Dan; Wang,Erkang; Wang,Jin
刊名	small
	2015
卷号	11 期号:19 页码:2333-2340
关键词	anion-sensitive gene delivery gold nanoparticles liposome pH-sensitive
通讯作者	li,d
英文摘要	gene therapy is a potential method for treating a large range of diseases. gene vectors are widely used in gene therapy for promoting the gene delivery efficiency to the target cells. here, gold nanoparticles (aunps) coated with dimethyldioctadecylammonium bromide (dodab)/dioleoylphosphatidylethanolamine (dope) are synthesized using a facile method for a new gene vector (dodab/dope-aunps), which possess 3- and 1.5-fold higher transfection efficiency than those of dodab-aunps and a commercial transfection agent, respectively. meanwhile, it is nontoxic with concentrations required for effective gene delivery. imaging and quantification studies of cellular uptake reveal that dope increases gene copies in cells, which may be attributed to the smaller size of aunps/dna complexes. the dissociation efficiency of dna from the endocytic pathway is quantified by incubating with different buffers and investigated directly in the cells. the results suggest that dope increases the internalization of aunps/dna complexes and promotes dna release from early endosomes for the vector is sensitive to the anionic lipid membrane and the decreasing ph along the endocytic pathway. the new vector contains the potential to be the new alternative as gene delivery vector for biomedical applications.
收录类别	SCI
语种	英语
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64594]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Du,Baoji,Tian,Li,Gu,Xiaoxiao,et al. Anionic Lipid, pH-Sensitive Liposome-Gold Nanoparticle Hybrids for Gene Delivery - Quantitative Research of the Mechanism[J]. small,2015,11(19):2333-2340.
APA	Du,Baoji,Tian,Li,Gu,Xiaoxiao,Li,Dan,Wang,Erkang,&Wang,Jin.(2015).Anionic Lipid, pH-Sensitive Liposome-Gold Nanoparticle Hybrids for Gene Delivery - Quantitative Research of the Mechanism.small,11(19),2333-2340.
MLA	Du,Baoji,et al."Anionic Lipid, pH-Sensitive Liposome-Gold Nanoparticle Hybrids for Gene Delivery - Quantitative Research of the Mechanism".small 11.19(2015):2333-2340.