Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat
	Liu, Caixiang 1; Ding, Feng 4; Hao, Fuhua 1; Yu, Men 1,5; Lei, Hehua 1; Wu, Xiangyu 1; Zhao, Zhengxi 6; Guo, Hongxiang 7; Yin, Jun 7; Wang, Yulan 1,3
刊名	SCIENTIFIC REPORTS
	2016-02-10
卷号	6
英文摘要	Pre-harvest sprouting (PHS) is a worldwide problem for wheat production and transgene antisense-thioredoxin-s (anti-trx-s) facilitates outstanding resistance. To understand the molecular details of PHS resistance, we analyzed the metabonomes of the transgenic and wild-type (control) wheat seeds at various stages using NMR and GC-FID/MS. 60 metabolites were dominant in these seeds including sugars, organic acids, amino acids, choline metabolites and fatty acids. At day-20 post-anthesis, only malate level in transgenic wheat differed significantly from that in controls whereas at day-30 post-anthesis, levels of amino acids and sucrose were significantly different between these two groups. For mature seeds, most metabolites in glycolysis, TCA cycle, choline metabolism, biosynthesis of proteins, nucleotides and fatty acids had significantly lower levels in transgenic seeds than in controls. After 30-days post-harvest ripening, most metabolites in transgenic seeds had higher levels than in controls including amino acids, sugars, organic acids, fatty acids, choline metabolites and NAD+. These indicated that anti-trx-s lowered overall metabolic activities of mature seeds eliminating pre-harvest sprouting potential. Post-harvest ripening reactivated the metabolic activities of transgenic seeds to restore their germination vigor. These findings provided essential molecular phenomic information for PHS resistance of anti-trx-s and a credible strategy for future developing PHS resistant crops.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	METABONOMIC ANALYSIS ; PHOSPHOLIPASE-D ; BARLEY-GRAIN ; THIOREDOXIN ; NMR ; PROTEIN ; EXPRESSION ; RESPONSES ; IDENTIFICATION ; DEHYDROGENASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000369741600001
公开日期	2016-04-26
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/9189]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Chinese Acad Sci, CAS Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Fudan Univ, Collaborat Innovat Ctr Genet & Dev Biol, State Key Lab Genet Engn, Metabon & Syst Biol Lab,Sch Life Sci, Shanghai 200438, Peoples R China 3.Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310058, Zhejiang, Peoples R China 4.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China 5.Wuhan Zhongke Metaboss Ltd, Wuhan 430074, Peoples R China 6.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China 7.Henan Agr Univ, Natl Engn Res Ctr Wheat, Zhengzhou 450002, Peoples R China
推荐引用方式 GB/T 7714	Liu, Caixiang,Ding, Feng,Hao, Fuhua,et al. Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat[J]. SCIENTIFIC REPORTS,2016,6.
APA	Liu, Caixiang.,Ding, Feng.,Hao, Fuhua.,Yu, Men.,Lei, Hehua.,...&Tang, Huiru.(2016).Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat.SCIENTIFIC REPORTS,6.
MLA	Liu, Caixiang,et al."Reprogramming of Seed Metabolism Facilitates Pre-harvest Sprouting Resistance of Wheat".SCIENTIFIC REPORTS 6(2016).