Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas
	Hong, Chen; Si, Yanxiao; Xing, Yi; Li, Yang
刊名	ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
	2015-07
卷号	22 期号:14 页码:10788-10799
关键词	Iron mine MiSeq sequencing Alpha diversity Bacterial phyla Potential resistance genera Heavy metal
英文摘要	Mine activities leaked heavy metals into surrounding soil and may affected indigenous microbial communities. In the present study, the diversity and composition of the bacterial community in soil collected from three regions which have different pollution degree, heavy pollution, moderate pollution, and non-pollution, within the catchment of Chao River in Beijing City, were compared using the Illumina MiSeq sequencing technique. Rarefaction results showed that the polluted area had significant higher bacterial alpha diversity than those from unpolluted area. Principal component analysis (PCA) showed that microbial communities in the polluted areas had significant differences compared with the unpolluted area. Moreover, PCA at phylum level and Matastats results demonstrated that communities in locations shared similar phyla diversity, indicating that the bacterial community changes under metal pollution were not reflected on phyla structure. At genus level, the relative abundance of dominant genera changed in sites with degrees of pollution. Genera Bradyrhizobium, Rhodanobacter, Reyranella, and Rhizomicrobium significantly decreased with increasing pollution degree, and their dominance decreased, whereas several genera (e.g., Steroidobacter, Massilia, Arthrobacter, Flavisolibacter, and Roseiflexus) increased and became new dominant genera in the heavily metal-polluted area. The potential resistant bacteria, found within the genera of Thiobacillus, Pseudomonas, Arthrobacter, Microcoleus, Leptolyngbya, and Rhodobacter, are less than 2.0 % in the indigenous bacterial communities, which play an important role in soil ecosystem. This effort to profile the background diversity may set the first stage for better understanding the mechanism underlying the community structure changes under in situ mild heavy metal pollution.
研究领域[WOS]	Environmental Sciences
WOS记录号	WOS:000357344300040
公开日期	2016-03-15
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/32600]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Hong, Chen,Si, Yanxiao,Xing, Yi,et al. Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2015,22(14):10788-10799.
APA	Hong, Chen,Si, Yanxiao,Xing, Yi,&Li, Yang.(2015).Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,22(14),10788-10799.
MLA	Hong, Chen,et al."Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 22.14(2015):10788-10799.