The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation

CORC > 上海神经科学研究所 > 中国科学院上海生命科学研究院神经科学研究所 > 神经所(总)

	The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation
	Lyu, JW; Yu, XY; He, LJ; Cheng, TL; Zhou, JJ; Cheng, C; Chen, ZF; Cheng, GQ; Qiu, ZL; Zhou, WH
刊名	MOLECULAR BRAIN
	2015
卷号	8 期号:1 页码:26-26
关键词	ISCHEMIC BRAIN-INJURY IN-VIVO PROLIFERATION NEUROGENESIS SUPPRESSION DELETION GENE
通讯作者	Zhou, WH (reprint author), Fudan Univ, Childrens Hosp, Dept Neonatol, 399 Wanyuan Rd, Shanghai 201102, Peoples R China.,zwhchfu@126.com
英文摘要	Background: The tumor suppressor gene Phosphatase and tensin homolog (PTEN) is highly expressed in neural progenitor cells (NPCs) and plays an important role in development of the central nervous system. As a dual-specificity phosphatase, the loss of PTEN phosphatase activity has been linked to various diseases. Results: Here we report that the protein phosphatase activity of Pten is critical for regulating differentiation of neural progenitor cells. First we found that deletion of Pten promotes neuronal differentiation. To determine whether the protein or lipid phosphatase activity is required for regulating neuronal differentiation, we generated phosphatase domain-specific Pten mutations. Interestingly, only expression of protein phosphatase-deficient mutant Y138L could mimic the effect of knocking down Pten, suggesting the protein phosphatase of Pten is critical for regulating NPC differentiation. Importantly, we showed that the wild-type and lipid phosphatase mutant (G129E) forms of Pten are able to rescue neuronal differentiation in Pten knockout NPCs, but mutants containing protein phosphatase mutant cannot. We further found that Pten-dependent dephosphorylation of CREB is critical for neuronal differentiation. Conclusion: Our data indicate that the protein phosphatase activity of PTEN is critical for regulating differentiation of NSCs during cortical development.
学科主题	Neurosciences
WOS标题词	Neurosciences & Neurology
收录类别	SCI
语种	英语
WOS记录号	WOS:000354159800001
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/3952]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Lyu, JW,Yu, XY,He, LJ,et al. The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation[J]. MOLECULAR BRAIN,2015,8(1):26-26.
APA	Lyu, JW.,Yu, XY.,He, LJ.,Cheng, TL.,Zhou, JJ.,...&Zhou, WH.(2015).The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation.MOLECULAR BRAIN,8(1),26-26.
MLA	Lyu, JW,et al."The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation".MOLECULAR BRAIN 8.1(2015):26-26.