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	Determination of several sugars in serum by high-performance anion-exchange chromatography with pulsed amperometric detection
	Cai, YQ; Liu, JS; Shi, YL; Liang, LN; Mou, SF
	2005
会议名称	17th International Ion Chromatograhy Symposium
会议日期	SEP 20-23, 2004
会议地点	Trier, GERMANY
关键词	sugar serum high-performance anion-exchange chromatography pulsed amperometric detection
英文摘要	In this paper, a sensitive, simple and direct method for simultaneous determination of glucose, ribose, isomaltose and maltose in serum sample by high-performance anion-exchange chromatography coupled with integrated pulsed amperometric detection was developed. The four target analytes were easily and completely separated on an anion-exchange column at a flow-rate of 0.25 mL/min by binary step gradient elution in about 16 min and the two eluents were deionized water and 500 mM sodium hydroxide, respectively. The separated four analytes were detected directly by using a gold electrode and quadruple-potential waveform integrated pulsed amperometry without derivatization. Under the optimized conditions, when the injection volume was 25 mu L, the detection limits (signal-to-noise ratio equal to 3) for glucose, ribose, isomaltose and maltose were 0.92, 7.50, 12.9 and 10.3 ng/mL, respectively. The calibration graphs of peak area for the four analytes were linear over two to three orders of magnitude with correlation coefficients greater than 0.998. R.S.D. of peak areas of the four analytes for five determinations were no more than 5.6%. The analytical method had been applied to the determination of glucose, ribose, isomaltose and maltose in real serum samples and good results with low relative standard deviation not more than 5.3% were obtained. The accuracy of the proposed method was tested by recovery measurements on spiked samples and good recovery results (98.1-107.9%) were obtained. (c) 2004 Elsevier B.V. All rights reserved.
ISSN号	0021-9673
内容类型	会议论文
源URL	[http://ir.rcees.ac.cn/handle/311016/24757]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Cai, YQ,Liu, JS,Shi, YL,et al. Determination of several sugars in serum by high-performance anion-exchange chromatography with pulsed amperometric detection[C]. 见:17th International Ion Chromatograhy Symposium. Trier, GERMANY. SEP 20-23, 2004.

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