Intestinal immune function, antioxidant status and tight junction proteins mRNA expression in young grass carp (Ctenopharyngodon idella) fed riboflavin deficient diet

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Intestinal immune function, antioxidant status and tight junction proteins mRNA expression in young grass carp (Ctenopharyngodon idella) fed riboflavin deficient diet
	Chen, Liang 1; Feng, Lin 1,2,3; Jiang, Wei-Dan 1,2,3; Jiang, Jun 1,2,3; Wu, Pei 1,2,3; Zhao, Juan 1; Kuang, Sheng-Yao 4; Tang, Ling 4; Tang, Wu-Neng 4; Zhang, Yong-An 5
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-11-01
卷号	47 期号:1 页码:470-484
关键词	Riboflavin Intestinal immune Tight junction Antioxidant Signaling molecules
ISSN号	1050-4648
英文摘要	This study investigated the effects of riboflavin on intestinal immunity, tight junctions and antioxidant status of young grass carp (Ctenopharyngodon idella). Fish were fed diets containing graded levels of riboflavin (0.63-10.04 mg/kg diet) for 8 weeks. The study indicated that riboflavin deficiency decreased lysozyme, acid phosphatase, copper/zinc superoxide dismutase, glutathione reductase and glutathione peroxidase activities, and contents of complement component 3 and reduced glutathione in the intestine of fish (P < 0.05). Meanwhile, riboflavin deficiency increased reactive oxygen species, malondialdehyde and protein carbonyl contents and catalase activity (P < 0.05) in the intestine of fish. Furthermore, real-time polymerase chain reaction analysis was used to investigate mRNA expression patterns and found that the mRNA levels of interleukin 10 and transforming growth factor beta 1, Occludin, zonula occludens 1, Claudin-b and Claudin-c, inhibitor protein kappa B alpha, target of rapamycin, ribosomal S6 protein kinase 1 and NF-E2-related factor 2, copper/zinc superoxide dismutase, glutathione peroxidase and glutathione reductase were decreased (P < 0.05) in the intestine of fish fed riboflavin-deficient diet. Conversely, the mRNA levels of tumor necrosis factor alpha, interleukin 1 beta, interleukin 8, nuclear factor kappa B p65, Ikappa B kinase beta, Ikappa B kinase gamma, Kelch-like-ECH-associated protein 1b, p38 mitogen-activated protein kinase, myosin light chain kinase and Claudin-12 were increased (P < 0.05) in the intestine of fish fed riboflavindeficient diet. In conclusion, riboflavin deficiency decreased immunity and structural integrity of fish intestine. The optimum riboflavin level for intestinal acid phosphatase activity of young grass carp was estimated to be 6.65 mg/kg diet. (c) 2015 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	NF-KAPPA-B ; ENZYME-ACTIVITIES ; JUVENILE JIAN ; VAR. JIAN ; GENE-EXPRESSION ; BARRIER FUNCTION ; EPINEPHELUS-COIOIDES ; SIGNALING MOLECULES ; GROWTH-PERFORMANCE ; OXIDATIVE STRESS
收录类别	SCI
语种	英语
WOS记录号	WOS:000365053300054
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27398]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Chen, Liang,Feng, Lin,Jiang, Wei-Dan,et al. Intestinal immune function, antioxidant status and tight junction proteins mRNA expression in young grass carp (Ctenopharyngodon idella) fed riboflavin deficient diet[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(1):470-484.
APA	Chen, Liang.,Feng, Lin.,Jiang, Wei-Dan.,Jiang, Jun.,Wu, Pei.,...&Liu, Yang.(2015).Intestinal immune function, antioxidant status and tight junction proteins mRNA expression in young grass carp (Ctenopharyngodon idella) fed riboflavin deficient diet.FISH & SHELLFISH IMMUNOLOGY,47(1),470-484.
MLA	Chen, Liang,et al."Intestinal immune function, antioxidant status and tight junction proteins mRNA expression in young grass carp (Ctenopharyngodon idella) fed riboflavin deficient diet".FISH & SHELLFISH IMMUNOLOGY 47.1(2015):470-484.