Aggregation and antigenicity of virus like particle in salt solution-A case study with hepatitis B surface antigen

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	Aggregation and antigenicity of virus like particle in salt solution-A case study with hepatitis B surface antigen
	Chen, Yi 1,2; Zhang, Yan 1; Quan, Can 3; Luo, Jian 1; Yang, Yanli 1; Yu, Mengran 1; Kong, Yingjun 1; Ma, Guanghui 1; Su, Zhiguo 1
刊名	VACCINE
	2015-08-20
卷号	33 期号:35 页码:4300-4306
关键词	Asymmetrical flow field-flow fractionation Virus-like particle Aggregation Antigenicity Hepatitis B surface antigen
ISSN号	0264-410X
英文摘要	The phenomenon of aggregation of virus-like particles (VLPs) in salt solution and the corresponding effect upon antigenicity was reported. Asymmetrical flow field-flow fractionation (AF4) combined with multi-angle laser light scattering (MALLS) was used to characterize the size and the aggregation behavior of hepatitis 13 surface antigen (HBsAg). The average diameter of HBsAg VLP was 22.8 +/- 0.4 nm and it tended to aggregate in salt solution to form large particles and the antigenicity changed accordingly. In 0-4 M NaCl solution, part of HBsAg molecules aggregated rapidly into oligomeric particles (OP), whose diameter distributed from 25 to 40 nm, and the antigenicity slightly decreased about 10%. The aggregation reaction is reversible. After removing NaCl, both size and antigenicity could recover to normal level (92-96%). By contrast, the aggregation process is more complicated in (NH4)(2)SO4 solution. Most of HBsAg particles aggregated into OP and further aggregated into polymeric particles (PP). The diameter of the PP could reach 40 to 140 nm. The concentration of (NH4)(2)SO4 had remarkable influence upon the rate of aggregation. When concentration of (NH4)(2)SO4 was below 1 M, most of HBsAg aggregated only into OP in 1 h. While with concentration of (NH4)(2)SO4 above 1 M, most of particles formed PP within 1 h. The aggregation process to PP was irreversible. After removing (NH4)(2)SO4, the large aggregates could not recover to normal particles and the remaining antigenicity was below 30%. (C) 2015 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Immunology ; Medicine, Research & Experimental
研究领域[WOS]	Immunology ; Research & Experimental Medicine
关键词[WOS]	FIELD-FLOW FRACTIONATION ; SIZE-EXCLUSION CHROMATOGRAPHY ; LASER-LIGHT SCATTERING ; QUANTITATIVE CHARACTERIZATION ; ELECTRON-MICROSCOPY ; VACCINE FORMULATION ; STABILIZATION ; DELIVERY ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000360870500012
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/19538]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Natl Inst Metrol, Div Chem, Beijing 100013, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yi,Zhang, Yan,Quan, Can,et al. Aggregation and antigenicity of virus like particle in salt solution-A case study with hepatitis B surface antigen[J]. VACCINE,2015,33(35):4300-4306.
APA	Chen, Yi.,Zhang, Yan.,Quan, Can.,Luo, Jian.,Yang, Yanli.,...&Su, Zhiguo.(2015).Aggregation and antigenicity of virus like particle in salt solution-A case study with hepatitis B surface antigen.VACCINE,33(35),4300-4306.
MLA	Chen, Yi,et al."Aggregation and antigenicity of virus like particle in salt solution-A case study with hepatitis B surface antigen".VACCINE 33.35(2015):4300-4306.