Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles
	Lu, WW; Wang, LH; Li, J; Zhao, Y; Zhou, Z; Shi, JY; Zuo, XL; Pan, D
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 页码:—
关键词	COLORIMETRIC DETECTION OPTICAL-PROPERTIES THERMAL-STABILITY NONTHIOLATED DNA GENE-REGULATION OLIGONUCLEOTIDE FUNCTIONALIZATION POLYNUCLEOTIDES NANOTECHNOLOGY HYBRIDIZATION
英文摘要	In recent years, poly adenine (polyA) DNA functionalized gold nanoparticles (AuNPs) free of modifications was fabricated with high density of DNA attachment and high hybridization ability similar to those of its thiolated counterpart. This nanoconjugate utilized poly adenine as an anchoring block for binding with the AuNPs surface thereby facilitated the appended recognition block a better upright conformation for hybridization, demonstrating its great potential to be a tunable plasmonic biosensor. It's one of the key points for any of the practical applications to maintaining stable conjugation between DNA oligonucleotides and gold nanoparticles under various experimental treatments. Thus, in this research, we designed a simple but sensitive fluorescence turn-on strategy to systematically investigate and quantified the dissociation of polyA DNA on gold nanoparticles in diverse experimental conditions. DNA desorbed spontaneously as a function of elevated temperature, ion strength, buffer pH, organic solvents and keeping time. What's more, evaluating this conjugate stability as affected by the length of its polyA anchor was another crucial aspect in our study. With the improved understanding from these results, we were able to control some of our experimental conditions to maintain a good stability of this kind of polyA DNA-AuNPs nanoconjugates.
收录类别	SCI
语种	英语
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/24549]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Lu, WW,Wang, LH,Li, J,et al. Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles[J]. SCIENTIFIC REPORTS,2015,5:—.
APA	Lu, WW.,Wang, LH.,Li, J.,Zhao, Y.,Zhou, Z.,...&Pan, D.(2015).Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles.SCIENTIFIC REPORTS,5,—.
MLA	Lu, WW,et al."Quantitative investigation of the poly-adenine DNA dissociation from the surface of gold nanoparticles".SCIENTIFIC REPORTS 5(2015):—.