Ion chromatographic analysis of tetracyclines using polymeric column and acidic eluent

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	Ion chromatographic analysis of tetracyclines using polymeric column and acidic eluent
	Ding, XJ; Mou, SF
刊名	JOURNAL OF CHROMATOGRAPHY A
	2000
卷号	897 期号:2015-01-02 页码:205-214
关键词	antibiotics tetracyclines
英文摘要	High-performance ion chromatography (HPIC) is first successfully used to analyze tetracycline antibiotics (TCs) in this work. The TCs are well separated on a solvent compatible polymeric cation-exchange column within 12 min. Isocratic elution with acetonitrile-hydrochloride is very advantageous for routine analysis. HPIC may be seen as a specific variant of the more common high-performance liquid chromatography (HPLC) for water-soluble and polar pharmaceuticals with low hydrophobicity. The detection limits (signal-to-noise ratio similar or equal to3:1) of oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), doxycycline (DC) are 10, 10, 20 and 20 mug l(-1), respectively. Samples are prepared by vortex mixing with an ethylenediaminetetraacetic acid disodium salt (Na(2)EDTA)-McIlvaine buffer (pH 4.0) solution and the mixture filtrates through a molecular weight cut-off filter. The method has been successfully applied to monitor the OTC removal rate through every reactor in the process of OTC manufacturing wastewater treatment by bio-chemical technology. It is also applicable to determine the TCs residues in milk and milk powder with satisfying results. (C) 2000 Elsevier Science B.V. All rights reserved.
WOS记录号	WOS:000165093000017
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/24121]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Ding, XJ,Mou, SF. Ion chromatographic analysis of tetracyclines using polymeric column and acidic eluent[J]. JOURNAL OF CHROMATOGRAPHY A,2000,897(2015-01-02):205-214.
APA	Ding, XJ,&Mou, SF.(2000).Ion chromatographic analysis of tetracyclines using polymeric column and acidic eluent.JOURNAL OF CHROMATOGRAPHY A,897(2015-01-02),205-214.
MLA	Ding, XJ,et al."Ion chromatographic analysis of tetracyclines using polymeric column and acidic eluent".JOURNAL OF CHROMATOGRAPHY A 897.2015-01-02(2000):205-214.