Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T)

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 微生物资源团队

	Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T)
	Yuan, Bo 1,3; Hu, Nan 1,2; Sun, Juan 2; Wang, Shi-An 1; Li, Fu-Li 1
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2012-12-01
卷号	96 期号:6 页码:1517-1526
关键词	Candida kutaonensis sp nov. Conserved motif Inulinase Purification Yeast
英文摘要	A novel extracellular exoinulinase was purified and characterized from a new yeast strain KRF1(T), and the gene encoding the enzyme was successfully cloned. The enzyme was stable at low pH between 3.0 and 6.5. The K (m) and V (max) values of the purified enzyme for inulin were 2.3 mg/mL and 4.8 mg/min, respectively. The optimum temperature of the inulinase was 50 A degrees C, and the enzyme remained 78 % of activity at 60 A degrees C for 2 h. The inulinase showed an amino acid sequence identity of 58 % to its closest homolog in Meyerozyma (Pichia) guilliermondii. In the secondary structure, the domain G (VMEVH) of the enzyme contained three unique residues (V, M, and H). Compared with previously reported inulinases, the enzyme from strain KRF1(T) displayed strong acid resistance, notable thermostability, and high affinity for the substrate of inulin. Based on sequence analysis of the 26S rDNA D1/D2 domain and phenotypic characterization, the yeast strain KRF1(T) was found to represent a novel anamorphic, ascomycetous yeast species. A complete description of the species is given and the name Candida kutaonensis sp. nov (type strain = KRF1(T) = AS 2.4027(T) = CBS 11388(T)) is proposed.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	CRYPTOCOCCUS-AUREUS G7A ; KLUYVEROMYCES-MARXIANUS ; JERUSALEM-ARTICHOKE ; ASPERGILLUS-NIGER ; PICHIA-GUILLIERMONDII ; MICROBIAL INULINASES ; ETHANOL FERMENTATION ; PURIFIED INULINASE ; SEQUENCE-ANALYSIS ; ENDO-INULINASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000311244800012
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6390]
专题	青岛生物能源与过程研究所_微生物资源团队
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao 266109, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Bo,Hu, Nan,Sun, Juan,et al. Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T)[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,96(6):1517-1526.
APA	Yuan, Bo,Hu, Nan,Sun, Juan,Wang, Shi-An,&Li, Fu-Li.(2012).Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T).APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,96(6),1517-1526.
MLA	Yuan, Bo,et al."Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp nov KRF1(T)".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 96.6(2012):1517-1526.