SEPARATION AND QUANTIFICATION OF WATER-SOLUBLE CELLULAR METABOLITES IN CLOSTRIDIUM THERMOCELLUM USING LIQUID CHROMATOGRAPHY-ISOTOPE DILUTION TANDEM MASS SPECTROMETRY

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	SEPARATION AND QUANTIFICATION OF WATER-SOLUBLE CELLULAR METABOLITES IN CLOSTRIDIUM THERMOCELLUM USING LIQUID CHROMATOGRAPHY-ISOTOPE DILUTION TANDEM MASS SPECTROMETRY
	Cui, Jiatao 1,3; Zhang, Jingtao 1; Zhu, Xinshu 1,3; Bai, Fali 2; Feng, Yingang 1; Guan, Wenna 2; Cui, Qiu 1
刊名	ANALYTICAL LETTERS
	2013-11-22
卷号	46 期号:17 页码:2767-2786
关键词	Clostridium thermocellum Ethanol tolerance Isotope dilution mass spectrometry Liquid chromatography-mass spectrometry Water-soluble metabolites
英文摘要	A new protocol for metabolomic studies was developed by combining liquid chromatography-tandem mass spectrometry and isotope dilution mass spectrometry with universal C-13 labeled internal standards from Escherichia Coli. The multiple reaction monitoring mode of mass spectrometry was used for quantification. Forty-five water-soluble intracellular metabolites, including 20 amino acids, 16 organic acids (primarily from the tricarboxylic acid cycle), and 9 cofactors, were measured and 34 of them were successfully quantified using the C-13-labeled internal standards. The limit of detection, limit of quantification, precision, and linearity of the methods were evaluated. The methods were applied to the quantitative analysis of intracellular metabolites extracted from wild-type and ethanol-adapted strains of Clostridium thermocellum cultivated with and without ethanol stress, and all 34 metabolites including all 9 cofactors were successfully quantified. Further multivariate data analyses of the metabolic differences between wild-type and ethanol-adapted strains were performed on the quantitative data, which can help elucidate the metabolic mechanism behind ethanol adaptation in C. thermocellum. Supplemental materials are available for this article. Go to the publisher's online edition of Analytical Letters to view the supplemental file.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	TRICARBOXYLIC-ACID CYCLE ; BIOLOGICAL SAMPLES ; HUMAN PLASMA ; QUANTITATIVE-DETERMINATION ; INTRACELLULAR METABOLITES ; ELECTROSPRAY-IONIZATION ; HILIC-MS/MS ; PHASE ; EXTRACTION ; METABOLOMICS
收录类别	SCI
语种	英语
WOS记录号	WOS:000325960300009
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/5996]
专题	青岛生物能源与过程研究所_代谢物组学团队
作者单位	1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Publ Lab Bioenergy & Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cui, Jiatao,Zhang, Jingtao,Zhu, Xinshu,et al. SEPARATION AND QUANTIFICATION OF WATER-SOLUBLE CELLULAR METABOLITES IN CLOSTRIDIUM THERMOCELLUM USING LIQUID CHROMATOGRAPHY-ISOTOPE DILUTION TANDEM MASS SPECTROMETRY[J]. ANALYTICAL LETTERS,2013,46(17):2767-2786.
APA	Cui, Jiatao.,Zhang, Jingtao.,Zhu, Xinshu.,Bai, Fali.,Feng, Yingang.,...&Cui, Qiu.(2013).SEPARATION AND QUANTIFICATION OF WATER-SOLUBLE CELLULAR METABOLITES IN CLOSTRIDIUM THERMOCELLUM USING LIQUID CHROMATOGRAPHY-ISOTOPE DILUTION TANDEM MASS SPECTROMETRY.ANALYTICAL LETTERS,46(17),2767-2786.
MLA	Cui, Jiatao,et al."SEPARATION AND QUANTIFICATION OF WATER-SOLUBLE CELLULAR METABOLITES IN CLOSTRIDIUM THERMOCELLUM USING LIQUID CHROMATOGRAPHY-ISOTOPE DILUTION TANDEM MASS SPECTROMETRY".ANALYTICAL LETTERS 46.17(2013):2767-2786.