Isolation and Expression Analysis of Three Types of -Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments
Shi, Chongli1,2; An, Meiling2,3,4; Miao, Jinlai2,3,4; He, Yingying2,4; Zheng, Zhou2,3,4; Qu, Changfeng2; Wang, Xixi2; Lin, Huan1; Liu, Junhong1
刊名MOLECULAR BIOTECHNOLOGY
2019-03
卷号61期号:3页码:200-208
关键词Chlamydomonas sp ICE-L qRT-PCR Bioinformatic analysis Carbonic anhydrase
ISSN号1073-6085
DOI10.1007/s12033-018-00152-4
英文摘要Carbonic anhydrases (CAs) are a class of zinc-containing metalloenzymes that can reversibly catalyse the hydration reaction of carbon dioxide. Antarctic algae are the most critical component of the Antarctic ecosystem; algae can enter the carbon cycle food chain by fixing carbon dioxide from the air. In this study, the complete open reading frames (ORFs) of CA1 (GenBank ID KY826431), CA2 (GenBank ID KY826432), and CA3 (GenBank ID KY826433), encoding CAs in the Antarctic ice microalga Chlamydomonas. sp. ICE-L, were successfully cloned using reverse transcription-polymerase chain reaction (RT-PCR). In addition, the expression patterns of CAs under blue light, under UV light, and in the dark were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The CA1, CA2, and CA3 ORFs encode proteins of 376, 430, and 419 amino acids, respectively. Phylogenetic analysis revealed that all amino acid sequences showed high homology with those of C. sp. ICE-L. There are six types of algal CAs; we hypothesised that the CAs studied here are most likely -CAs. Expression analysis showed that the transcription level of the CAs was influenced by both UV light and blue light. These findings provide additional insight into the molecular mechanisms of CAs and will accelerate the development of CAs for applications in agriculture and environmental governance.
资助项目Ningbo Public Service Platform for High-Value Utilization of Marine Biological Resources[NBHY-2017-P2]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
出版者HUMANA PRESS INC
WOS记录号WOS:000458164100004
内容类型期刊论文
源URL[http://ir.fio.com.cn:8080/handle/2SI8HI0U/10921]  
专题业务部门_海洋生态研究中心
通讯作者Miao, Jinlai
作者单位1.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China
2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
4.Qingdao Univ, Med Coll, Qingdao 266071, Peoples R China
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Shi, Chongli,An, Meiling,Miao, Jinlai,et al. Isolation and Expression Analysis of Three Types of -Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments[J]. MOLECULAR BIOTECHNOLOGY,2019,61(3):200-208.
APA Shi, Chongli.,An, Meiling.,Miao, Jinlai.,He, Yingying.,Zheng, Zhou.,...&Liu, Junhong.(2019).Isolation and Expression Analysis of Three Types of -Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments.MOLECULAR BIOTECHNOLOGY,61(3),200-208.
MLA Shi, Chongli,et al."Isolation and Expression Analysis of Three Types of -Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments".MOLECULAR BIOTECHNOLOGY 61.3(2019):200-208.
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