Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth
Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu; Wei Du
SourceSensors and Actuators B-Chemical
4296
Volume255Pages:735-744
DOI10.1016/j.snb.2017.08.088
Rank2
SubtypeArticle
English AbstractC. elegans is a leading model organism for studying the basic mechanisms of lifespan extension by dietary restriction (DR). Progress has been limited, however, by the lack of an automated system for quantitative analysis of this effect of bacterial diet. In this work, multifunctional logarithmic gradient-customizing microfluidic device, which takes advantage of hydrodynamics, was developed to long-term maintain worm culture on the chip with parallel live imaging and generate logarithmic concentration gradient of bacteria. We successfully employed this platform to investigate the longevity and stress response of the worms in response to various concentrations of bacterial food. The results indicated that the lifespan extension of the worms was induced by DR when bacteria density was 108 cells mL-1 and further dilutions would reduce the lifespan, which proposed a new dietary restriction regime in C. elegans. In addition, imaging analysis showed that DR regulated the subcellular localization DAF-16 in a way different from starvation that reduced lifespan of the worms. The platform allows not only horizontal studies of C. elegans in response to various environments over a wide range of concentrations, but also multiparameter evaluation of longevity in the worms with high temporal resolution, providing clues to better understand the aging process and help age-related drug screen.
WOS KeywordCAENORHABDITIS-ELEGANS ;  MICROFLUIDIC DEVICE ;  QUANTITATIVE-ANALYSIS ;  DEVELOPMENTAL-STAGES ;  LIFE-SPAN ;  GENETICS ;  PLATFORM ;  CHEMOTAXIS ;  LONGEVITY ;  PATHWAYS
WOS Research AreaChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
Language英语
WOS IDWOS:000414151800088
Funding OrganizationNational Natural Science Foundation of China ; National Key R&D Program of China
Content Type期刊论文
源URL[http://ir.qibebt.ac.cn/handle/337004/9869]  
Collection青岛生物能源与过程研究所_单细胞中心
Corresponding AuthorWei Du
Recommended Citation
GB/T 7714
Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,Wei Du. Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth[J]. Sensors and Actuators B-Chemical,4296,255:735-744.
APA Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,&Wei Du.(4296).Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth.Sensors and Actuators B-Chemical,255,735-744.
MLA Anle Ge, Liang Hu, Xixian Wang, Jinchi Zhu, Xiaojun Feng, Wei Du∗, Bi-Feng Liu,et al."Logarithmic bacterial gradient chip for analyzing the effects of dietaryrestriction on C. elegans growth".Sensors and Actuators B-Chemical 255(4296):735-744.
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