Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

doi:10.1016/j.snb.2017.08.088

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	Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth
	Ge, Anle 1; Hu, Liang 1; Wang, Xixian 1,2,3; Zhu, Jinchi 1; Feng, Xiaojun 1; Du, Wei 1; Liu, Bi-Feng 1
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018-02-01
卷号	255 页码:735-744
关键词	Microfluidic Device Caenorhabditis Elegans Logarithmic Bacterial Gradient Dietary Restriction Lifespan Extension
DOI	10.1016/j.snb.2017.08.088
文献子类	Article
英文摘要	C. elegans is a commonly used model organism for studying the basic mechanisms of lifespan extension by dietary restriction (DR). Progress has been limited, however, by the lack of an automated system for quantitative analysis of this effect of bacterial diet. In this work, multifunctional logarithmic gradient customizing microfluidic device, which takes advantage of hydrodynamics, was developed to long-term maintain worm culture on the chip with parallel live imaging and generate logarithmic concentration gradient of bacteria. We successfully employed this platform to investigate the longevity and stress response of the worms in response to various concentrations of bacterial food. The results indicated that the lifespan extension of the worms was induced by DR when bacteria density was 108 cells mL(-1) and further dilutions would reduce the lifespan, which proposed a new dietary restriction regime in C. elegans. In addition, imaging analysis showed that DR regulated the subcellular localization DAF-16 in a way different from starvation that reduced lifespan of the worms. The platform allows not only horizontal studies of C. elegans in response to various environments over a wide range of concentrations, but also multiparameter evaluation of longevity in the worms with high temporal resolution, providing clues to better understand the aging process and help age-related drug screen. (C) 2017 Elsevier B.V. All rights reserved.
WOS关键词	CAENORHABDITIS-ELEGANS ; MICROFLUIDIC DEVICE ; QUANTITATIVE-ANALYSIS ; DEVELOPMENTAL-STAGES ; LIFE-SPAN ; GENETICS ; PLATFORM ; CHEMOTAXIS ; LONGEVITY ; PATHWAYS
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
WOS记录号	WOS:000414151800088
资助机构	National Natural Science Foundation of China(21475049 ; National Key R&D Program of China(2016YFF0100801) ; NIH National Centre for Research Resources ; 31471257 ; 21275060)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9737]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Huazhong Univ Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Key Lab Biomed Photon,Syst Biol Theme,Dept Biomed, MOE,Wuhan Natl Lab Optoelect,Coll Life Sci & Tech, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Ge, Anle,Hu, Liang,Wang, Xixian,et al. Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,255:735-744.
APA	Ge, Anle.,Hu, Liang.,Wang, Xixian.,Zhu, Jinchi.,Feng, Xiaojun.,...&Liu, Bi-Feng.(2018).Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth.SENSORS AND ACTUATORS B-CHEMICAL,255,735-744.
MLA	Ge, Anle,et al."Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth".SENSORS AND ACTUATORS B-CHEMICAL 255(2018):735-744.